Karlsson has missed nine games with a lower-body injury and has not suited up for the Sharks since Jan.16.
The former Norris Trophy winner recorded 31 points over 25 games prior to the injury. Karlsson ranks fourth among defenseman with 0.91 points per game, and 10th in ice time while logging 24:59 a night.
The Sharks managed to maintain their strong play despite Karlsson's absence, going 6-3 and winning six of their last seven games without him.
San Jose occupies the second seed in the Pacific Division heading into Saturday's action.